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- Capillary electrophoresis
- Analysis of Proteins by Capillary Electrophoresis
- Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.
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In order to effectively detect these ultra-low abundance analytes among the endogenous high abundance interfering species, a combination of sample separation method with mass spectrometry MS is essential for a high-sensitivity and selectivity analysis. Although the resolution of gas or liquid-phase based separations are relatively lower as compared to MS, they both provide an effective way to simplify the sample mixture and alleviate the ion suppression effect during the ionization step prior to the MS analysis [ 2 ]. Capillary electrophoresis CE is considered as a promising alternative separation technique to liquid chromatography LC , due to its high separation efficiency, broad sample compatibility, relatively short analysis time and low sample consumption. The separation mechanism of CE is based on charge-to-size ratio differences of analytes, which is different from the LC separation mechanism. Combining LC with CE separation can often provide complementary information on the composition of a complex biological sample.
Analysis of Proteins by Capillary Electrophoresis
Capillary electrophoresis CE is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. Very often, CE refers to capillary zone electrophoresis CZE , but other electrophoretic techniques including capillary gel electrophoresis CGE , capillary isoelectric focusing CIEF , capillary isotachophoresis and micellar electrokinetic chromatography MEKC belong also to this class of methods. Additionally, analytes may be concentrated or "focused" by means of gradients in conductivity and pH. The instrumentation needed to perform capillary electrophoresis is relatively simple. A basic schematic of a capillary electrophoresis system is shown in figure 1. The system's main components are a sample vial, source and destination vials, a capillary, electrodes , a high voltage power supply , a detector, and a data output and handling device. The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution.
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EN English Deutsch. Your documents are now available to view. Confirm Cancel. Jochen Pauwels and Ann Schepdael. Versita Article Carbon nanotubes in capillary electrophoresis, capillary electrochromatography and microchip electrophoresis Jochen Pauwels, Ann Schepdael
Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis. July ; Analytical Methods 6(15)
Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.
Medical Center Dr. This review article describes the significant recent advances in the analysis of proteins by capillary and microchip electrophoresis during the period from mid to early This review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. The protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics. If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using Copyright Clearance Center.
PL-2 High throughput protein assays using microchip electrophoresis Robert Kennedy. Monday, September 30 th. KL-1 Fabrication of structured magnetic metallic nano-platelets for bio-analytical applications Frantisek Foret.
Protocol DOI: This chapter describes the basic principles of protein analysis by capillary electrophoresis, and provides an overview of the literature and thus a comprehensive summary of special topics in this field.
Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis
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